Figure 4.
Effect of AMPA/kainate, NMDA, and GABAA receptor pharmacological inhibition on synaptic responses evoked in SP and WM cells. Panels correspond to the cell type (SP or WM) indicated at the top of each column. A–C, Effects of CNQX alone on synaptic responses evoked at −70 mV. Representative examples of averaged evoked synaptic responses from an SP (A) and a WM (B) cell in aCSF (control; large black traces) and in CNQX (small gray traces). Summary of effects of CNQX on normalized peak evoked synaptic responses at −70 mV (C). D–F, Effect of APV alone on evoked synaptic currents at −70 mV. Representative examples of averaged evoked synaptic responses from an SP (D) and a WM (E) cell in aCSF (control; black traces) and in APV (gray traces). Summary of effects of APV on normalized peak evoked synaptic currents at −70 mV (F). G–I, Effect of APV and CNQX on evoked synaptic currents at −70 mV. Small currents remain in some cells at −70 mV after blockade of AMPA/kainate receptors with CNQX. Representative examples of average evoked synaptic responses from an SP (G) and a WM (H) cell in CNQX alone (black traces) and in CNQX/APV (gray traces). Summary of effect of APV/CNQX (vs CNQX alone) on normalized peak evoked synaptic responses at −70 mV (I). J, K, Effects of bicuculline on amplitude of average peak evoked synaptic responses of SP (J) and WM (K) cells. The peak responses are plotted for each individual cell tested in the control (Pre-Bicuc) condition and the experimental (Peri-Bicuc) condition. The control responses varied in their amplitude ranges as the bicuculline was applied after a no-drug control condition (aCSF), in conjunction with APV after application of APV alone, or in conjunction with CNQX/APV after application of CNQX/APV. *p < 0.05; **p < 0.01.