Figure 5.

dnCAV3 inhibits estradiol regulation of L-type calcium channel-dependent CREB phosphorylation. A, Estradiol attenuation of L-type calcium channel-dependent CREB phosphorylation (F = 34.52) was abolished after transfection of EYFP–dnCAV3. Conversely, EYFP–dnCAV3 had no effect on estradiol-induced CREB phosphorylation. B, Transfection of dnCAV3 had no effect on APDC-induced attenuation of depolarization-induced CREB phosphorylation (F = 126.0), suggesting that estradiol signaling was being disrupted upstream of mGluR2/3. 17βE, 17β-Estradiol.