Figure 4.
Interactions and affinities of Tat-NR2B9c to its PDZ-binding targets. A, Titration of Tat-NR2B9c at concentrations ranging from 0.0001 to 100 μm against the indicated PSD-95 PDZ domain constructs. Bars represent the mean ± SD of quadruplicate experiments. B, Determination of the EC50 of Tat-NR2B9c for binding PSD-95d1 (left) and PSD-95d2 (right). Symbols represent the mean ± SD of duplicate experiments. C, Interaction profile of Tat-NR2B9c against the panels of PDZ domains displaying interactions over background (2 PDZ arrays of 4 shown). For each pair of panels, titration of 0.007 μm Tat-NR2B9c (top) and 10 μm Tat-NR2B9c-AA (bottom) are shown. Bar pairs represent two individual interactions on separate microtiter plates. Hits not consistent between plates are repeated (interactions are generally classified for a given peptide concentration as 2× background and <20% SD).