Figure 1.
Generation of CLN3 reporter mice. A, Targeting construct and strategy for homologous recombination. B, Representative Southern blot from Cln3+/+ and Cln3lacZ/+ genomic DNA using the probe indicated by the shaded box A. C, Representative genomic PCR results from Cln3+/+, Cln3lacZ/+, and Cln3lacZ/lacZ mice (2 mice per group) using the primer sets depicted in A. D, RT-PCR results for CLN3 (left) and HPRT as control (right) using cDNA isolated from Cln3+/+, Cln3lacZ/+, and Cln3lacZ/lacZ mice as indicated. E, QPCR analysis for relative abundance of Cln3 and lacZ transcripts using the same cDNA samples as in D; Cln3 and lacZ transcripts were below detection levels in Cln3lacZ/lacZ and Cln3+/+ samples, respectively. Hc, Hippocampus; Cx, cortex; Cb, cerebellum; BS, brainstem; W, water.