Table 1.
Characteristics of wild-type and N-acylation-deficient Gα t rods
| Dark current (pA) | Ioa (photons/μm2) | Integration time (ms) | Dim flash τrec (ms) | Time to peak (ms) | Elementary amplitude (pA) | Bright flash τDb (ms) | |
|---|---|---|---|---|---|---|---|
| G2A−/− | 13.9 ± 0.7 (33) | 341.1 ± 24.2 (28) | 407 ± 30 (27) | 487 ± 32 (26) | 102 ± 6 (30) | 0.23 ± 0.03 (11) | 672 ± 98 (6) |
| G2A+/− | 10.4 ± 1.1 (11) | 65.6 ± 16.3 (7) | 259 ± 32 (9) | 245 ± 27 (10) | 116 ± 9 (10) | 0.58 ± 0.15 (5) | 184 ± 14 (6) |
| Wild type | 10.8 ± 0.9 (15) | 53.0 ± 3.9 (14) | 324 ± 30 (13) | 239 ± 19 (13) | 116 ± 7 (14) | 0.51 ± 0.07 (10) | 224+ 11 (10) |
Numbers in parentheses represent n.
aFlash strength that elicited a half-maximal response.
bDominant time constant for recovery of saturating flash responses.