Figure 4.
L-IFD induces overexpression of the NR2B subunit of NMDAR in the hippocampus. A–C, Basal expression of genes encoding different NMDAR subunits in hippocampal (A), somatosensory (B), and perirhinal (C) cortices of AL and L-IFD mice as assessed by semiquantitative RT-PCR. The GAPDH mRNA served as an internal control. The graphs represent the relative presence of specific PCR products in AL (black bars) and L-IFD (gray bars) mice. The NMDAR NR2B/2A subunit ratio in each area for AL and IFD mice is also shown. D, Photomicrographs and immunohistochemical analyses of the NR2B expression pattern in the hippocampus of AL and L-IFD mice. The graphs represent the densitometric analysis of NR2B expression in the different areas of hippocampus (n = 5 animals per group in all tests). s luc, Stratum lucidum; mol, molecular layer; l mol, lacunosum moleculare layer; Molec, molecular layer; Lac mol, lacunosum moleculare layer; O.D., optical density. *p ≤ 0.01; **p ≤ 0.01; ***p ≤ 0.001.