Figure 2.

Role of IL-1RI signaling in the potentiation of hypoxic neuronal injury by IL-1β in vitro. A, B, Mixed cortical cell cultures were treated with 1 ng/ml IL-1β for 20–24 h in the presence or absence of rIL-1ra (10–1000 ng/ml; A) or anti-IL-1RI (0.1–100 μg/ml; B), washed, and then deprived of oxygen (5 h). The percentage of total neuronal cell death was determined 20–24 h later. An asterisk indicates values significantly greater than hypoxia alone, whereas # denotes a significant diminution of the IL-1β-mediated increase in injury (IL-1β) as determined by one-way ANOVA followed by a Student–Newman–Keuls t test. Significance was assessed at p < 0.05 (n = 3–9 cultures pooled from 2–3 different experiments).