Figure 2.

MDMA induced lipid peroxidation in whole-brain mitochondria and the protective effect of selegiline. Lipid peroxidation was determined by measuring MDA equivalents, using the TBA assay. Animals were killed 14 d after exposure to: MDMA (4 × 10 mg/kg), selegiline (Sel.) (2 mg/kg) plus MDMA (4 × 10 mg/kg), saline (isovolumetric saline), or selegiline (2 mg/kg). Selegiline was administered 30 min before exposure to MDMA. Columns represent mean + SEM, expressed in nanomoles MDA equivalents per milligram of protein for each experimental group (n = 10 for control and MDMA; n = 6 for MDMA plus selegiline and selegiline). Animals exposed to MDMA presented significantly higher values of MDA equivalents than all other groups (*p < 0.001, one-way ANOVA followed by a post hoc Scheffé's test).