Figure 2.
Localization of S-opsin and retinal morphology in 3-week-old mice. A, C, E, G, Localization of S-opsin (green) visualized by immunofluorescence microscopy of frozen retinal sections. B, D, F, H, Retinal morphology of the corresponding genotype. A, S-opsin reactivity in endogenous cones of the control rho+/− retina. B, Transgenic S-opsin-1D4rho+/− labeled with S-opsin (green) and rhodopsin (red) show colocalization (yellow). Retinal morphology is mostly normal in these genotypes (B, D). S-opsin-1D4 and S-opsin are correctly localized to the outer segments when they are expressed in the rho−/− background (E, G). Disorganized outer segment structures are seen in these mice (F, H).