Figure 1.

REST occupies SACO-identified RE1 sites in vivo. A, A sequence logo representing the PWM used to identify RE1 sites in the genome. B, Histograms of GST-RE1 offsets showing that the sequences surrounding the SACO sites are enriched only for RE1s and not for permutations of the RE1. C, ChIP showing REST binding to SACO-identified RE1 sites in TCMK1 kidney cells. The chromosome (Chr) number and position of each site is indicated on the left (University of California, Santa Cruz, Mm5 build). Immunoprecipitations were performed with antibodies directed against either REST-N or REST-C or with a rabbit IgG antibody. Sonicated genomic DNA served as a positive control for each RE1 site (Input).