Figure 6.

Discovery of expanded RE1 sites. A, Sequence logo representation of the PWM used to identify expanded RE1 sites in the genome. NNN represents 3–9 bp that comprise the insertion. B, Quantitative PCR of chromatin immunoprecipitations in TCMK1 kidney cells showing REST binding to expanded RE1 sites. Fold changes in DNA immunoprecipitated with antibodies directed against REST-N or REST-C or with a control rabbit IgG antibody are shown relative to sonicated genomic DNA (Input). The chromosome (Chr) number and position of the expanded RE1 sites are indicated (University of California, Santa Cruz, Mm5 build), as are the number of nucleotides that comprise each insertion (Split3 indicates that 3 bp make up the insertion). Error bars represent the SD from three independent experiments.