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. 2007 Jun 20;27(25):6607–6619. doi: 10.1523/JNEUROSCI.0790-07.2007

Figure 5.

Figure 5.

Glutamate transporters are expressed by astrocytes in both gray and white matter. A, Confocal fluorescence image of the CA1 region of the hippocampus from a P24 GLT-1–eGFP BAC mouse, incubated with an antibody (ab) against GFAP, showing the distribution of eGFP (green) and GFAP (blue). B, C, Higher-magnification images of the region delimited by the white box in A, showing expression of eGFP (B) by GFAP+ astrocytes (C). D, Whole-cell recording of glutamate transporter currents elicited in a stratum radiatum astrocyte (identified expression of eGFP) in response to photolysis of MNI–d-aspartate (125 μm). The red trace shows the response recorded in the presence of the glutamate transporter antagonist TBOA (300 μm). P34 GLT-1–eGFP BAC mouse. E, Confocal fluorescence image of the corpus callosum from a P24 GLT-1–eGFP BAC mouse showing the distribution of eGFP in the corpus callosum. F, G, Higher-magnification images of the region delimited by the white box in E, showing expression of eGFP (F) by GFAP+ astrocytes (G) in this white matter region. H, Whole-cell recording of glutamate transporter currents elicited in a corpus callosum astrocyte (identified expression of eGFP) in response to photolysis of MNI–d-aspartate (125 μm). The red trace shows the response recorded in the presence of the glutamate transporter antagonist TBOA (300 μm). P34 GLT-1–eGFP BAC transgenic mouse.