Figure 2.

VEGF is downregulated in cells overexpressing G93A SOD1 protein. A, Western blot of U251 tet-on clones expressing wild-type or G93A mutant SOD1 FLAG fusion proteins. Ectopic proteins are detected when the cells are stimulated with Dox (1.0 μg/ml). B, VEGF RNA levels in clone 1 for WT and G93A SOD1 as determined by qRT-PCR. pTRE is a clone expressing empty vector and U251 (p) is the parent U251 tet-on cell line. Results are the mean (±SE) of five to nine independent Dox stimulations for each clone, analyzed in triplicate. *p < 0.001 compared with pTre and U251 (p) controls and p < 0.01 compared with the WT clone.