Table 2.
Antibodies used, source, and dilution
| Antigen (clone) | Type | Species | Clonality | Dilution | Source |
|---|---|---|---|---|---|
| Nestin (RAT401) | IgG1 | Mouse | Monoclonal | 1:1000 | Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA |
| Islet ½ | IgG | Mouse | Monoclonal | 1:200 | |
| BrdU (G3G4) | IgG1 | Mouse | Monoclonal | 1:5000 | |
| NF200 (N52) | IgG1 | Mouse | Monoclonal | 1:1000 | Sigma |
| βIII-Tubulin (SDL0.3D10) | IgG2b | Mouse | Monoclonal | 1:500 | |
| GFAP (G-A-5) | IgG1 | Mouse | Monoclonal | 1:500 | |
| SMA (1A4) | IgG2a | Mouse | Monoclonal | 1:500 | |
| BrdU (Bu20a) | IgG | Mouse | Monoclonal | 1:200 | DakoCytomation |
| GFAP | IgG | Rabbit | Polyclonal | 1:1000 | |
| S100β | IgG | Rabbit | Polyclonal | 1:1000 | |
| DCX | IgG | Rabbit | Polyclonal | 1:1000 | Abcam, Cambridge, MA |
| Goat | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA | |||
| PGP9.5 | IgG | Rabbit | Polyclonal | 1:1000 | Chemicon |
| p75NTR (MC192) | IgG1 | Mouse | Monoclonal | 1:1000 | |
| p75NTR (#9650)a | IgG | Rabbit | Polyclonal | 1:2000 | Gift from Prof. M. V. Chao, New York University, New York, NY |
| P75NTR (MLR3)b | IgG2a | Mouse | Monoclonal | 1:500 | Gift from Dr. M.-L. Rogers, Flinders University, Adelaide, South Australia, Australia |
| NSE | IgG | Rabbit | Polyclonal | 1:2000 | Polysciences, Warrington, PA |
aCharacterized by Dr. Chao's Laboratory (Huber and Chao, 1995).
bCharacterized previously by Rogers et al. (2006).