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. 2019 Jul 25;10:914. doi: 10.3389/fphys.2019.00914

Figure 2.

Figure 2

Effects of c-met inhibitor PHA-665752 on muscle regeneration. After CTX injury, mice were i.p. injected with 20 mg/kg of PHA-665752 on a daily basis until sacrificed. CTX injured TAs were analyzed 7 days postinjury. (A) Effect on muscle weight. Muscle mass was normalized with the weight of mice. PHA, PHA-665752. ns, not significant, **p < 0.01, ***p < 0.001 (one-way ANOVA), n = 4 per group. (B) H&E staining of regenerating muscle. Crosses (x) and asterisks (*) indicate necrotic and phagocyted myofibers, respectively. Scale bars, 200 μm. (C) Effect on cross-sectional areas of muscle fibers. Mean value of area sizes is indicated in the graph. **p < 0.01, ***p < 0.001 (one-way ANOVA), n = 4 per group. (D) Quantification of necrotic or phagocyted fibers expressed as a percentage of total myofibers. At least 300 muscle fiber areas were counted per sample. **p < 0.01, ***p < 0.001 (unpaired student’s t test), n = 4 per group. All data are presented as mean ± SEM. See also Supplementary Figure S2.