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A triple-labeling in situ hybridization analysis for T1Rs and PLCβ2 in medaka fish taste buds. A–D, In situ hybridization was performed using horizontal sections of medaka fish gill raker, palate, and pharynx with antisense riboprobes for T1R3, PLCβ2 and T1R1 (A), T1R2a (B), T1R2b (C), or T1R2c (D). Signals are shown in pseudocolor as indicated at the bottom of the panel, and merged images for each result are shown in the right panels. Scale bar, 20 μm.
