Table 2.
Primary antibodies used
| Host | Antigen | Target tissue/structures | Dilution | Source |
|---|---|---|---|---|
| Sheep | NGF | 1:1000 | Neuromics (Medina, MN) | |
| Chicken | BDNF | 1:1000 | Neuromics | |
| Goat | NT-3 | 1:100 | Neuromics | |
| Rabbit | NT-4 | 1:1000 | Chemicon (Temecula, CA) | |
| Goat | Human Fc | 1:100 | Sigma-Aldrich Canada | |
| Mouse | Cd11b (Ox-42) | Microglia | 1:500 | Sigma-Aldrich Canada |
| Rabbit | GFAP | Astrocytes | 1:1000 | Dako Canada (Mississauga, Ontario, Canada) |
| Mouse | RECA-1 | Blood vessels | 1:1000 | Serotec (Raleigh, NC) |
| Guinea pig | VGLUT1 | Mechanosensory/ corticospinal terminals | 1:4000 | Chemicon |
| Goat | CTB | Mechanosensory terminals | 1:2000 | Cedarlane |
| Rabbit | SERT | Serotonergic axons | 1:1200 | Immunostar (Hudson, WI) |
GFAP, Glial fibrillary acidic protein; RECA-1, rat endothelial cell antigen 1; SERT, serotonin transporter.