Figure 5.

Ligand-induced neurite outgrowth in Neuro2A coexpressing GFRα2b with other GFRα isoforms. GFRα2b was stably coexpressed with GFRα2a (GFRα2a + GFRα2b) or GFRα2c (GFRα2c + GFRα2b) (A) or GFRα1a (GFRα1a + GFRα2b) (B). Cells were treated with or without GDNF or NTN (50 ng/ml) for 3 d in 0.5% serum-containing medium. Retinoic acid (RA; 5 μm) differentiated all the transfectants efficiently. Experiments have been repeated twice with three independent clones, with similar results. Significant differences in the percentage of cells bearing neurites between ligand stimulated and control were calculated using the paired Student's t test (**p < 0.002; *p = 0.05). Error bars indicate mean ± SD of triplicate measurements.