Figure 5.

The FIGQY motif is required for IS detergent resistance of L1/NgCAM. A, B, Localization of L1 (red in A), ankG (green), and ankB (red in B) in non-extracted control neurons (left) and after Triton X-100 extraction (TE) (right). MAP2 staining is in blue. IS are indicated by arrows. Scale bar, 50 μm. C, D, Localization of NgCAM (C) and NgCAMΔFIGQY (D) in nonextracted control neurons (left) and Triton X-100-extracted (TE) neurons (middle). Anti-NgCAM staining (red) is overlaid over ankG (green) in the right panels to indicate position of ankG-positive IS (arrows). Dendrites are indicated with arrowheads.