Figure 6.

No alteration in amyloidogenic APP processing in BRI-Aβ40/Tg2576 mice. RIPA-soluble forebrain extracts from 8-month-old BRI-Aβ40/Tg2576, Tg2576, BRI-Aβ40, and non-Tg littermates were analyzed by Western blotting. A, B, Blots were probed with 82E1 (anti-Aβ1-16) (A) or CT20 (anti-APP C-terminal 20 amino acids) (B), stripped, and reprobed with anti-β actin to assess loading. C, D, The relative levels of C99 (C) and APP (D) after normalization to β-actin were equivalent between Tg2576 mice and BRI-Aβ40/Tg2576 mice, indicating that there was no alteration in the processing of APP in the bitransgenic mice (p > 0.1). C99 and APP levels were equivalent between non-Tg and BRI-Aβ40 mice that express only endogenous mouse APP (p > 0.1). n = 5 mice per genotype. For statistical analysis, see Materials and Methods.