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TIMP-3 decreases surface levels of ADAM-10. COS7 cells were transfected with ADAM-10 cDNA (HA tagged on its N terminus) and either TIMP-3 or a control vector. Surface proteins were labeled with a water-soluble biotinylating agent and isolated using avidin beads. Surface levels of ADAM-10 were measured from the precipitated proteins using anti-HA antibody. Total ADAM-10 levels were measured in complete cell lysates. Cotransfection with TIMP-3 decreased surface levels of ADAM-10.
