Figure 3.

TBS-induced p-cofilin immunoreactivity is normal in Fmr1-KOs. A, B, Laser confocal photomicrographs show p-cofilin-immunoreactivity in proximal CA1 stratum radiatum of hippocampal slices from WT (A) and Fmr1-KO (FX; B) mice that received either baseline low-frequency stimulation (lfs) or five TBSs; slices were collected 7 min after stimulation. Scale bar, 1 μm. C, Bar graph shows the number of p-cofilin-immunoreactive (ir) puncta (mean ± SEM) per 100 μm² for fields receiving lfs (open bars) or TBS (closed bars) in WT and Fmr1-KO slices. Two-way ANOVA demonstrated a significant effect of TBS (p = 0.00096), but no effect of genotype on p-cofilin-ir puncta counts. Thus, numbers of p-cofilin-ir puncta were significantly greater in slices that received TBS than in those that received lfs for both WTs (**p = 0.0019, t test; lfs, n = 3 mice; TBS, n = 3 mice) and Fmr1-KOs (*p = 0.033, t test; lfs, n = 3 mice; TBS, n = 3 mice).