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. 2007 Oct 3;27(40):10685–10694. doi: 10.1523/JNEUROSCI.2624-07.2007

Figure 4.

Figure 4.

Fmr1-KO mice show normal activity-dependent actin polymerization in dendritic spines. Acute hippocampal slices prepared from Fmr1-KO or WT mice were processed for in situ Alexafluor 568-phalloidin labeling of filamentous actin after electrophysiological recording in hippocampal region CA1. LTP was induced by TBS; control slices received baseline, low-frequency stimulation (lfs). A, Photomicrographs of Fmr1-KO (left) and WT (right) hippocampal slices showing representative phalloidin labeling in the field of afferent stimulation in CA1 stratum radiatum after TBS or lfs. Scale bar, 10 μm. B, Plot summarizes group mean (±SEM) numbers of densely phalloidin-labeled spine-like puncta per sample field for control/lfs (white bars) and TBS (black bars) slices. As indicated, TBS induced similar increases in the numbers of densely labeled spines between genotypes (**p < 0.01 vs respective control group, Tukey's HSD after ANOVA). C, High-magnification photomicrographs show examples of densely phalloidin-labeled dendritic spines in CA1 stratum radiatum from Fmr1-KO and WT slices receiving TBS. Scale bar, 1 μm.