Table 1.

Summary of disease course and cell parameters in WT→WT and WT→IL-4^−/− BM chimera mice^a

	WT→WT	WT→IL-4−/−
Day of onsetb	14.3 ± 0.7	11.6 ± 0.5c
Day 18 average score	1.9 ± 0.3	2.9 ± 0.1d
Number of mice	9	13
Total number of mononuclear cells (×103)	310 ± 21	640 ± 90e
Absolute number of lymphocytes (×103)f	109 ± 9	274 ± 26c
Absolute number of resting CD45low microglial cells (×103)f	115 ± 17	143 ± 22
Absolute number of activated CD45high microglial cells (×103)f	47 ± 11	127 ± 19e
Absolute number of peripheral macrophages (×103)f	37 ± 4	74 ± 7e

^aLethally irradiated WT or IL-4^−/− C57BL/6 mice were transplanted with BM from (C57BL/6xB10.PL)F₁ mice and allowed to reconstitute for 8 weeks, and EAE was induced by immunization with MOG_35–55 peptide. The data shown are the average ± SE of three experiments with three to five mice per group observed on day 18 after EAE induction.

^bGraded disease score as described in Materials and Methods.

^cp < 0.005 when compared to WT→WT.

^dp < 0.01 when compared to WT→WT.

^ep < 0.05 when compared to WT→WT.

^fAbsolute numbers were determined by multiplying the total cell count obtained by counting on a hemocytometer by the percentage of lymphocytes, resting and activated microglial cells, and peripheral macrophages determined by flow cytometry as for Figure 3 and then dividing by the number of mice in each group.