Figure 1.

GLAST::CreERT2 knock-in mice are a useful tool to study adult neurogenesis. a, Schematic drawing of the experimental design depicting the induction of recombination in GLAST::CreERT2/R26R mice (3 months of age) and the analysis at different time points thereafter. b, c, Micrographs depicting immunostaining for β-galactosidase reporter activity (green) and GFAP (red) in the SGZ of the DG (b, b') and SEZ (c, c'). The histograms in d depict the quantification of reporter+ neuroblasts (DCX+) among all DCX+ neuroblasts at different times after tamoxifen application. Error bars indicate SEM. e, Fluorescence micrograph depicting β-galactosidase+ (green) neuroblasts labeled for DCX (red) 9 months after TM induction in the SEZ (white arrow) and the RMS (white arrowhead). Scale bars: b, c, e, 50 μm; b', c', 10 μm. N, Number of animals; LV, lateral ventricle; UB, upper blade; LB, lower blade in the DG.