Figure 2.

Cells in the Ptf1a lineage do not contain MF neurons. A, Schematic drawing that indicates the injection sites of Fluorogold and retrogradely labeled areas in the caudal hindbrain (left) and the spinal cord (right). Fluorogold was injected into four sites of the cerebellum: anterior and posterior sites of hemisphere and vermis. B–P, Double immunostaining with anti-Fluorogold (green) and β-gal (red) antibodies in the rectangular regions indicated in A. Merged images are also shown. Scale bars, 40 μm. Sp5, Spinal trigeminal nucleus; CC, Clarke's column (dorsal nucleus).