Table 1.
Antibodies, source, and dilutions
Antibodies | Source | Dilutions |
---|---|---|
Polyclonal goat anti-human C1q | DakoCytomation (Glostrup, Denmark) | 1:100,a 1:1000b |
Polyclonal rabbit anti-human C1r | DakoCytomation | 1:100,a 1:1000b |
Polyclonal rabbit anti-human C1s | DakoCytomation | 1:100,a 1:1000b |
Polyclonal rabbit anti-human C3c | DakoCytomation | 1:750 |
Polyclonal rabbit anti-human C4c | DakoCytomation | 1:100 |
Polyclonal rabbit anti-rat C9 | B. P. Morgan | 1:300 |
Polyclonal rabbit anti-human MBP | DakoCytomation | 1:100,a 1:50c |
Monoclonal mouse anti-human | Sternberger (Lutherville, MD) | 1:1000 |
Phosphorylated neurofilament (SMI31 clone) | ||
Monoclonal mouse anti-rat CD11b (ED7 clone) | C. Dijkstra (VU University Medical Centre, Amsterdam, The Netherlands) | 1:100 |
Monoclonal mouse anti-rat CD68 (ED1 clone) | Serotec (Oxford, UK) | 1:100,a 1:50c |
Monoclonal mouse anti-rat Pan (Ki-M2R clone) | Acris (Hiddenhausen, Germany) | 1:100 |
Polyclonal rabbit anti-cow S100 | DakoCytomation | 1:400 |
aDilution used in the colorimetric immunostaining.
bDilution used in the Western blotting.
cDilution used in the fluorescence immunostaining.