Discharge patterns of ventral interneurons at 7 and 14 DIV. A, Current-clamp recordings from spinal interneurons in organotypic cultures at 7 DIV. The 500 ms depolarizing current commands induced different discharge patterns that identified four cell categories: tonic, adapting, delay, and irregular. B, Depolarizing commands induced different discharge patterns recorded from spinal interneurons at 14 DIV to identify four cell categories: tonic, adapting, delay, and transient. C, D, Bar charts illustrate the probability distribution (expressed as percentage of sampled population) of each cell type at 7 DIV (C) and 14 DIV (D) (see Results for details).