Figure 4.

E4031 effects on network excitability of spinal slices at 7 and 14 DIV. A, Top, Bursting activity in control solution recorded from a 7 DIV interneuron located in the ventral area of a spinal slice in culture. Bottom, The application (10 min) of E4031 (1 μm) prolonged burst duration with a reduction in burst frequency. B, Representative tracings of spontaneous activity, recorded at 14 DIV in control condition (top trace) and in the presence of 1 μm E4031 (10 min; bottom trace). Note that at 14 DIV, E4031 failed to evoke any increase in firing activity and that E4031 significantly increased firing frequency at 7 DIV only.