Figure 7.

Butanone enhancement experiment with the odor of food instead of food. The chemotaxis indexes of wild-type (N2), osm-9, and osm-9;olrn-2 animals preexposed to butanone and the odor of food are shown, together with those of naive animals, animals preexposed to butanone and food, and animals exposed to butanone alone. The osm-9 mutant was used to reduce the effect of butanone adaptation, which hindered the detection of chemotaxis enhancement by preexposure to butanone and the odor of food. The osm-9;olrn-2 double mutant was used as a negative control of the osm-9 mutant. In all of these experiments, the amount of butanone and the time length for preexposure were exceptionally 0.24 μl and 60 min to reduce the effect of butanone adaptation. Twelve plates were used for “N2, Butanone + Odor of food,” 9 plates for “N2, Butanone − Food,” 13 plates for “osm-9, Naive,” and 15 plates for “osm-9, Butanone + Odor of food.” Five to 15 plates were used for other data. *p < 0.005, comparing wild-type animals preexposed to butanone and the odor of food with those preexposed to butanone alone. **p < 0.05, comparing osm-9 animals preexposed to butanone and the odor of food with naive osm-9 animals. The error bars indicate SEM.