Table 1.
Summary of the midbrain-r1 defects observed in Fgfr compound mutant embryos
| Genotype | Phenotype |
||
|---|---|---|---|
| E9.5 | E12.5 | E18.5 | |
| Fgfr1cko | Boundary defect | DA disorganized; DR reduced | Ve, IC deleted; DA, LC disorganized; DR reduced |
| Fgfr2cko | n.a. | − | − |
| Fgfr3null | n.a. | − | − |
| Fgfr1cko;Fgfr2cko | Apoptosis, mispatterning, no IsO gene expression dorsally | DA decreased; DR deleted | Cerebellum, SC, IC, DA, LC, III, IV, DR deleted |
| Fgfr1cko;Fgfr3null | n.a. | n.a. | Ve, IC deleted; DA, LC disorganized; DR reduced |
| Fgfr2cko;Fgfr3null | n.a. | n.a. | − |
| Fgfr1cko;Fgfr2cko;Fgfr3null | Apoptosis, mispatterning, no IsO gene expression | DA decreased; DR deleted | Cerebellum, PPT, SC, IC, DA, LC, III, IV, DR deleted |
III, Oculomotor nucleus; IV, trochlear nucleus; DR, serotonergic neurons of the dorsal raphe; IC, inferior colliculus of the midbrain; LC, locus ceruleus; PPT, posterior pretectum; SC, superior colliculus of the midbrain; Ve, vermis of the cerebellum (medial cerebellum); n.a., not analyzed;–, no phenotypical defects observed.