Figure 5.

Exposure to rotenone accelerates PPL1 dopaminergic neurodegeneration and locomotor deficits in transgenic parkin R275W mutant flies. A, Representative confocal microscopy images showing TH-positive (red) dopaminergic neurons in the PPL1 cluster of 15-d-old, rotenone-treated, transgenic flies expressing wild-type (WT) or mutant parkin species, as indicated. B, Bar graph showing the number of dopaminergic (DA) neurons in different clusters of the various fly species treated with rotenone, as depicted by different shades shown in the figure (*p < 0.05, **p < 0.01, Student's t test; n = 10). C, Bar graph showing the climbing scores of the various rotenone-treated flies at different days after eclosion, as depicted by different shades shown in the figure (*p < 0.05, **p < 0.01 Student's t test; n > 20) (genotype: Ddc-Gal4/+; UAS-hParkin/+, control Ddc-Gal4/+).