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. 2007 Aug 8;27(32):8616–8627. doi: 10.1523/JNEUROSCI.5041-06.2007

Figure 8.

Figure 8.

Cl− conductance in CR cells. A, Decline of [Cl−]i determined in gramicidin-perforated patch-clamp experiments after complete blockade of NKCC1 with 50 μm bumetanide. Circles represent mean ± SEM of six experiments. The maximal Cl− efflux rate was calculated from the tangent aligned to the fit at the first data point (dashed line). B, Current–voltage relationship of membrane currents observed in the continuous presence of 0.2 μm TTX and 1 mm CsCl (black triangle). Although the combined application of the Cl− channel blockers DIDS (375 μm) and 9-AC (1 mm) had little effect on membrane currents (gray diamonds), blockade of ligand-gated Cl− channels with GBZ (3 μm) and strychnine (Strych; 30 μm) reduced membrane currents at depolarized potentials (open circle). C, IV plot of current difference (ΔI) between control and 9-AC/DIDS containing bathing solutions. Slope Cl− conductance was estimated between −80 and −60 mV. D, IV plot of current difference (ΔI) between control and GBZ/strychnine containing bathing solutions. Slope Cl− conductance was estimated between −80 and −60 mV.