AFI and absorption signal time courses in response to brief electrical stimulation in mouse. A–D, Time course of a single pixel in mouse visual cortex in response to electrical stimulation demonstrating autofluorescence signal (420–490 nm excitation filter; 520+ nm emission filter) (A), blue absorption signal (420–490 nm excitation filter, no emission filter) (B), green absorption signal (530–550 nm excitation filter; no emission filter) (C), and red absorption signal (600–620 nm excitation filter; no emission filter) (D). Each time course is from a single pixel and is the average of 22 cycles of stimulation. Error bars are SEM.