Figure 7.

Induction of C3 in microglial cultures. A–D, Neonatal mouse microglial cell cultures under phase (A, C) and labeled with two microglial markers, Iba1 (B) and CD11b (D). E, Quantitative real-time PCR shows strong upregulation of C3 mRNA 24 h after treatment with LPS (10 μg/ml) and TNF-α (10 ng/ml) but not IL-1β (10 ng/ml). E–H, Complement C3 protein is upregulated in the presence of LPS (10 μg/ml; G) and TNF-α (10 ng/ml; H) compared with control (F) after 24 h. DAPI staining for nuclei (blue) is shown in all merged images. Scale bar, 50 μm. *p < 0.05 (t test).