Figure 4.

Iqgap1- null mice show accumulation of neural progenitors. A, B, Wild-type and Iqgap1-null mice from the same litter were given injections of BrdU three times (once per hour) and killed 1 h after the last injection. A, Representative double immunostaining for BrdU (green) and PSA-NCAM (red) on sagittal sections in the proximal RE (a, c) and the RMS (b, d) of wild-type (a, b) and Iqgap1-null (c, d) mice. Arrowheads point to BrdU+ cells with neural progenitor features. Scale bars: a, c, 20 μm; b, d, 50 μm. B, Quantitative analysis of the percentage of BrdU+/PSA-NCAM− and BrdU+/PSA-NCAM+ cells in wild-type and Iqgap1-null mice. Results represent the average of four independent experiments. A total of 8001 BrdU+ cells in wild-type animals and 7615 BrdU+ cells in Iqgap1-null mice were analyzed. Significant differences from the corresponding wild-type counterpart were determined by Student's t test (***p < 0.005). Error bars indicate SEM.