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. 2007 Apr 25;27(17):4530–4540. doi: 10.1523/JNEUROSCI.0772-07.2007

Figure 3.

Figure 3.

Reversibility of BoNT/E effects. A, Representative VEP responses from both hemispheres of animals injected with BoNT/E at P14. Age of recording is indicated on the left. Note reduced VEP amplitude and increased latency in the treated side at P28. Responses in the injected side are completely recovered by P35. CONTRA, Contralateral; IPSI, ipsilateral. B, Immunoblotting for cleaved SNAP-25 on protein extracts from the visual cortex at different times after BoNT/E injection. Cleaved SNAP-25 is no longer detectable 21 d after BoNT/E. cl. S-25, Cleaved SNAP-25; β-tub, β-tubulin (internal standard). C, Intact and BoNT/E-truncated SNAP-25 at different times after one single injection of BoNT/E at P14. Cortical protein extracts were separated and blotted, and filters were probed with an antibody raised against the N-terminal domain of SNAP-25 that allows simultaneous visualization of intact (S-25) and BoNT/E-cleaved SNAP-25 (cl. S-25). β-tub, β-Tubulin (internal standard). D, Densitometric analysis of intact (black circles) and BoNT/E-cleaved (gray circles) SNAP-25. BoNT/E effects are stable for ∼1 week and decline thereafter, being completely off by P35. Error bars indicate SE and, when not seen, are within the symbol.