Figure 5.
A–F, Expression of Homer in Drosophila adult brain. Immunofluorescence staining using an anti-Homer antibody on frozen head sections of w(CS10) (A, C, E, F) and homerR102 (B, D) is shown. A, Drosophila Homer was expressed in the optic lobes (OL) and most central brain neuropil but not at detectable levels in the peduncles (P) of the mushroom bodies. C, Intense staining was observed in the protocerebral bridge (PB). Ca, Calyx. E, Magnified view of A. Staining in the central complex included the ellipsoid body (EB) and fan-shaped body (FB). F, Staining was also observed in the antennal lobes (AL). The failure to observe staining in homerR102 sections B and D at levels approximating those shown in A and C provide evidence for the specificity of the antisera.