Table 2.
Summary of inducible HSP70 expression in select brain tumor cell lines (relative to unstressed cells or to non-HSP expression such as actin)
| Cell line | HSP70 expression (Western blot) |
Cell surface HSP70 expression (flow cytometry) |
Extracellular HSP70 release (ELISA) |
|||
|---|---|---|---|---|---|---|
| Constitutive | Heat stress | Constitutive | Heat stress | Constitutive | Heat stress | |
| D54MG derived from line A172, adult malignant | + | ++ | + | ++ | + | ++ |
| glioma | ||||||
| D392MG adult malignant glioma | +/− | +++ | ++ | +++ | ++ | +++ |
| D341MED pediatric medulloblastoma | ++ | +/− | + | ++ | + | +++ |
| SMA560 murine anaplastic astrocytoma, syngeneic to VM/Dk strain | +/− | ++++ | +/− | ++ | + | ++++ |
+/−, Detectable expression; +, moderate expression; ++ → ++++, high to very high expression.