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Generation of Kir4.1 cKO mice. A, Targeting construct for the recombinant Kir4.1f/f mouse line. neo/tk selection cassette was removed by crossing Kir4.1f/f and FLPeR mice. LA, Left arm homology; RA, right arm homology. B, Construct for the transgenic hGFAP–Cre line. mP1, Poly(A) signal from mouse protamine1 gene. C, Kir4.1 Western blot from P20 littermate WT, Kir4.1f/f, Kir4.1+/−, and Kir4.1 cKO brain and kidney tissue. Blotting for β-actin was used as a loading control.
