Table 1.
Summary of antibodies
| Antigen | Antibody (code and type) |
Dilution | Application | Source |
|---|---|---|---|---|
| Primary | ||||
| α6 (α6β1) | GoH3, RtM | 1:3000 | DIF | Camon, Wiesbaden, Germany |
| αM (αMβ2) | 5C6, RtM | 1:6000 | DIF | Camon |
| αX (αXβ2) | N418, HmM | 1:100 | DIF | Endogen, Hamburg, Germany |
| B7.2 | 09271D, RtM | 1:3000 | DIF | BD Pharmingen, Hamburg, Germany |
| CGRP | α-CGRP, RbP | 1:400 | IHC | Peninsula, Belmont, CA |
| CD44 | α-CD44, RtM | 1:5000 | IHC, DIF | Millipore, Billerica, MA |
| Galanin | RGG-7141, RbP | 1:400 | IHC | Peninsula |
| GFAP | Z334, RbP | 1:6000 | IHC, DIF | Dako, Hamburg, Germany |
| IBA1 | α-IBA1, RbP | 1:6000 | DIF | Dr. Imai, Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan |
| ICAM1 | α-ICAM1, RtM | 1:3000 | DIF | Camon |
| MHC1 | ER-HR52, RtM | 1:250 | DIF | BMA Biomedicals, Augst, Switzerland |
| TGFβRII | sc-400, RbP | 1:2000 | IHC | Santa Cruz Biotechnology, Santa Cruz, CA |
| Secondary and tertiary |
||||
| Goat Ig | FITC-cj α-Gt Ig, DkP | 1:100 | DIF | Dianova, Hamburg, Germany |
| Hamster Ig | FITC-cj α-Hm Ig, GtP | 1:100 | DIF | Dianova |
| Rat Ig | Biotinylated α-Rt Ig, GtP | 1:100 | IHC | Vector Laboratories |
| Rat Ig | FITC-cj α-Rt Ig, GtP | 1:100 | DIF | Dianova |
| Rabbit Ig | Biotinylated α-Rb Ig, | 1:100 | IHC | Vector Laboratories |
| GtP | ||||
| Rabbit Ig | Biotinylated α-Rb Ig, | 1:100 | DIF | Dianova |
| DkP |
RtM, Rat monoclonal; HmM, hamster monoclonal; RbP, rabbit polyclonal; DkP, donkey polyclonal; FITC-cj, fluorescein isothiocyanate conjugated; DIF, double immunofluorescence.