Table 1.
Primary antibodies used for dual-labeling immunohistochemistry
| Genotype | Primary antibodies | Concentration | Species | Source |
|---|---|---|---|---|
| GAD65–GFP | PV | 1:5000 | Goat | Swant (Bellinzona, Switzerland) |
| CR | 1:5000 | Goat | Swant | |
| nNOS | 1:25,000 | Sheep | Dr. P. Emson (The Babraham Institute, Cambridge, UK) | |
| GAD67–GFP | PV | 1:5000 | Goat | Swant |
| CR | 1:5000 | Goat | Swant | |
| nNOS | 1:25,000 | Sheep | Dr. P. Emson | |
| Wild type | PV | 1:1000 | Mouse | Sigma |
| vs | ||||
| nNOS | 1:25,000 | Sheep | Dr. P. Emson | |
| vs | ||||
| PV | 1:1000 | Mouse | Sigma | |
| vs | ||||
| CR | 1:5000 | Goat | Swant | |
| vs | ||||
| CR | 1:5000 | Goat | Swant | |
| vs | ||||
| nNOS | 1:1500 | Mouse | Santa Cruz Biotechnology (Santa Cruz, CA) |