Figure 1.

CD200 is expressed on neurons and is reduced in chronic neuroinflammatory conditions. A, Double immunofluorescence for CD200 (i) and βIII-tubulin (ii) and a merged image (iii) in cultured neurons. B, Double immunofluorescence for MHCII (i) and CD200R (ii) and a merged image (iii) in mixed cultured glia treated with Aβ. C, CD200 protein expression decreases with age as shown by Western blot (*p < 0.05; n = 13). D, Fluorescent images of CD200 in the dentate gyrus of young (i) and aged (ii) animals. E, Age-related increase in the expression of MHCII mRNA (***p < 0.001; n = 13). F, Images of MHCII staining in the hippocampal CA1 region of young (i) and aged (ii) animals. Scale bars: A, B, 20 μm; D, 10 μm; F, 50 μm. Error bars indicate ±SEM.