Figure 3.

Increase in AMPAR-mediated current at individual synapses in the absence of NR2B. A, B, Example whole-cell voltage-clamp traces from NR2B sister cultures (WT and NR2B−/−) recorded in the presence of gabazine, TTX, and cyclothiazide to isolate mEPSCs demonstrate the increase in “miniature” AMPAR-mediated EPSC amplitudes in the absence of NR2B protein. C, Cumulative data showing the range and mean amplitude ± SEM of mEPSCs in the two conditions; recordings from sister cultures are delineated by the connecting lines. D, The same dataset plotted by cumulative histogram for all the recorded events from each genotype showing the right shift, toward higher amplitude, in the knock-out neurons. E, Ensemble average mEPSCs from single neurons of each genotype (at 11 DIV) demonstrate this increased amplitude in the absence of any change in current kinetics.