Figure 5.

Enhancement of PSC amplitude in NR2B−/− neurons is the result of a cell-autonomous mechanism. A, B, NR2B WT neurons from GFP-expressing animals (β-actin GFP; WT-GFP) were cocultured with either WT or NR2B−/− cells (50%/50% cocultures). This allowed acquisition of simultaneous recordings from WT-GFP neurons and nearby neurons, either NR2B WT or NR2B−/− in culture (position of recording pipettes is highlighted in A). Simultaneous whole-cell recordings of AMPA-mediated sEPSCs (V _hold, −65 mV) are shown for a neuron pair in a WT:WT-GFP coculture (C) and an NR2B−/−:WT-GFP coculture (D). E, Cumulative data from coculture recordings showed a significant increase in the amplitude of sEPSCs in NR2B−/− neurons recorded simultaneously with WT neurons in the same cultures (mean ± SEM). F, Unitary sEPSC event traces with collective average traces overlaid show the strong increase in amplitude of NR2B−/− responses (black) compared with simultaneously recorded WT-GFP responses (green) in the absence of any change in response kinetics (inset).