Influence of MEK inhibition on ERK1/2 and CREB phosphorylation in vivo. A, MEK inhibitor SL327 (300 μmol/kg, i.p.) pretreatment (15 min) blocked A-582941 (1.0)-induced and basal (tonic) ERK1/2 phosphorylation in mouse cingulate cortex (*p < 0.05 vs nontreated control; +
p < 0.05 vs A-582941 alone; n = 6 per treatment). B, MEK inhibitor SL327 (300 μmol/kg, i.p.) pretreatment (15 min) only produced a partial nonsignificant attenuation of A-582941-induced CREB phosphorylation, suggesting that other non-MAPK pathways may be involved in α7 agonism-mediated CREB phosphorylation (*p < 0.01 vs vehicle-treated controls). Data are represented as mean ± SEM.