Figure 8.

Cadherin-11 can immunoprecipitate with Trio in E15 ION and fMN extracts, and its expression is not affected in the mis-organized ION and fMN. In A, Western blot illustrating the presence of Cadherin-11, N-Cadherin, Nr-CAM, Notch, Abl, and DCC in E15 ION extracts. Cadherin-11 only could interact with Trio. Abl and Cadherin-11 were also expressed in control fMN extracts at E15, and only Cadherin-11 was immunoprecipitated with Trio (B). At E17, Cadherin-11 transcripts were expressed in the rostral ION in WT mice (C), in particular in the DAO and PO (C). Cadherin-11 remained expressed in trio mutant mice, in the mis-organized DAO at E17 (arrow in D). fMNn maintained Cadherin-11 synthesis in the absence of trio (F), but transcripts remained expressed randomly in the entire fMN but mainly expressed in the intermediate and in part of the lateral fMN subnucleus in wild-type fMN (E). Analysis of the amount of Cadherin-11 proteins in both WT and trio mutant mice by Western blot (G) reveals that the amount of Cadherin-11 is the same in WT and trio^−/− hindbrains. Scale bars: (in C) C, D, 240 μm; (in E) E, F, 130 μm.