Table 1.
Antibodies used for immunohistochemistry on human brain sections
| Antigen/antibody | Isotype | Commercial source | Concentration used | Secondary and tertiary used |
|---|---|---|---|---|
| MICA/B | Mouse IgG2a | BD Biosciences | 25 μg/ml | Goat anti-mouse biotin |
| Streptavidin-Cy3 | ||||
| APC-CC1 | Mouse IgG1 | Calbiochem (La Jolla, CA) | 2 μg/ml | Goat anti-mouse FITC |
| MAG | Mouse IgG1 | Millipore (Temecula, CA) | 5 μg/ml | Goat anti-mouse biotin |
| Streptavidin-Cy3 | ||||
| GFAP-Alexa 488 | Mouse IgG1 | Invitrogen | 10 μg/ml | NONE |
| CD8-allophycocyanin | Mouse IgG1 | BD Biosciences | 3 μg/ml | Rabbit anti-APC |
| Donkey anti-rabbit FITC | ||||
| Goat anti-mouse biotin | n.a. | Dako (Carpinteria, CA) | 9 μg/ml | n.a. |
| Streptavidin-Cy3 | n.a. | Jackson ImmunoResearch (West Grove, PA) | 3.6 μg/ml | n.a. |
| Rabbit anti-APC | n.a. | Biomeda (Cedarlane, Burlington, Ontario, Canada) | 10 μg/ml | n.a. |
| Donkey anti-rabbit FITC | n.a. | Jackson ImmunoResearch | 15 μg/ml | n.a. |
| Goat anti-mouse FITC | n.a. | Biosource | 14.8 μg/ml | n.a. |
n.a., Not applicable.