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. 2007 Oct 24;27(43):11510–11521. doi: 10.1523/JNEUROSCI.3077-07.2007

Figure 2.

Figure 2.

SpH is expressed in presynaptic boutons of pyramidal neurons in the hippocampus. a, The histogram of fluorescent intensities (ΔF) in single boutons before and after application of 50 mm NH4Cl in stratum radiatum and stratum oriens of acute hippocampal slices from spH21 and TV-46 transgenic mice. *p < 0.05. b, c, Diagrams of hippocampal slice preparations from spH21 (b) and TV-46 (c) transgenic mice. Stimulating electrodes (black coils) were placed either in the stratum radiatum (1) or stratum pyramidale (2). The spH-expressing neurons (green) and non-spH-expressing neurons (black) send their axons to the stratum radiatum and stratum oriens. d, e, Changes in spH fluorescence as a function of time in the stratum radiatum (red) and stratum oriens (green) before, during, and after 10 Hz stimulation of the stratum radiatum and stratum pyramidale in hippocampal slices from spH21 (d) and TV-46 (e) mice. Each trace is the average fluorescence from 21 to 29 boutons (4–5 slices). The fast decline in spH fluorescence in experiments using TV-46 mice is attributed to enhanced photobleaching of spH. TV-46 mice express low levels of spH; therefore, stronger laser excitation must be used in those slices. Calibration: vertical, 2%; horizontal, 2 s.