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Two-photon excitation images from neocortex showing fluorescent layer 5B pyramidal cells with bundling apical dendrites. A, Dendritic bundles in motor cortex were visualized with in vivo two-photon microscopy. B, Fluorescently labeled layer 5B pyramidal neurons located in cell clusters with apical dendrites extending in bundles were also visualized with two-photon microscopy in the acute somatosensory cortex slice used for electrophysiological recordings.
